主要成就
荣誉
2007年 湖南省科技进步一等奖；
2010年 上海市医务职工科技创新能手;
2012年 上海市‘讲理想、比贡献’活动科技标兵”称号
学术报告
多次受邀在英国、德国、法国、韩国以及国内会议上作学术报告：
1，2012年分别受邀至日内瓦和南非参加世界卫生组织组织的流感病毒相关会议；
2，2010年2月，应邀参加世界卫生组织在日内瓦举办的甲流防控会议，并代表中国疫苗生产企业作相关报告；
3，2010年1月, 作为大陆特派代表参加两岸共同防治H1N1新型流感研讨会并作题为甲型H1N流感病毒疫苗研究的报告。
论文
2005年到上海所任现职以来国际杂志发表论文目录（75篇）：（标*为通讯作者）
1. He B, Chang H, Liu Z, Huang C, Liu X, Zheng D, Fang F, Sun B, Chen Z*. Infection of influenza virus NA vaccinated mice with homologous influenza virus leads to strong protection against heterologous influenza viruses. J Gen Virol. 2014 Aug 28
2. Huang C, Wang H, Wu S, Chang H, Liu L, Peng B, Fang F, Chen Ze*. Comparison of multiple DNA vaccines for protection against cytomegalovirus infection in BALB/c mice.Virol J. 2014 Jun 5;11(1):104. [Epub ahead of print]
3. Wang S, Dong J, Chai W, Li F, Wang S, Sun B, Chen Z*. Immunogenicity of a monovalent 2009 influenza A (H1N1) vaccine in infants: randomized, observer-masked, single-center clinical study. Springerplus. 2014 Jul 31
4. Wang B, Liu Z, Chen Q, Gao Z, Fang F, Chang H, Chen J, Xu B, Chen Z*. Genotype diversity of H9N2 viruses isolated from wild birds and chickens in Hunan Province, China. PLoS One. 2014 Jun 30;9(6)
5. Fan X, Hashem AM, Chen Z, Li C, Doyle T, Zhang Y, Yi Y, Farnsworth A, Xu K, Li Z, He R, Li X, Wang J. Targeting the HA2 subunit of influenza A virus hemagglutinin via CD40L provides universal protection against diverse subtypes. Mucosal Immunol. 2014 Jul 23
6. Hashem AM, Gravel C, Chen Z, Yi Y, Tocchi M, Jaentschke B, Fan X, Li C, Rosu-Myles M, Pereboev A, He R, Wang J, Li X. CD40 ligand preferentially modulates immune response and enhances protection against influenza virus. J Immunol. 2014 Jul 15;193(2):722-34.
7. Han Q, Chang C, Li L, Klenk C, Cheng J, Chen Y, Xia N, Shu Y, Chen Z, Gabriel G, Sun B, Xu K. Sumoylation of influenza A virus nucleoprotein is essential for intracellular trafficking and virus growth. J Virol. 2014 Aug;88(16):9379-90
8. Liu Z, Xu B, Chen Q, Chen Z*. Complete Genome Sequence of a Mixed-Subtype (H5N1 and H6N6) Avian Influenza Virus Isolated from a Duck in Hunan Province, China. Genome Announc. 2014 Apr 24;2(2).
9. Zhang H, Li Y, Chen J, Chen Q, Chen Z*. Perpetuation of H5N1 and H9N2 Avian Influenza Viruses in Natural Waters. J Gen Virol. 2014 Mar 26.
10. Wang H, Yao Y, Huang C, Fu X, Chen Q, Zhang H, Chen J, Fang F, Xie Z, Chen Z*. An adjuvanted inactivated murine cytomegalovirus (MCMV) vaccine induces potent and long-term protective immunity against a lethal challenge with virulent MCMV. BMC Infect Dis. 2014 Apr 11
11. Liu Z, Xu B, Chen Q, Chen Z*. Complete Genome Sequence of a Mixed-Subtype (H5N1 and H6N6) Avian Influenza Virus Isolated from a Duck in Hunan Province, China. Genome Announc. 2014 Apr 24;2(2)
12. Zhang H, Chen Q, Chen Z*. A simple and efficient method for detecting avian influenza virus in water samples. J Virol Methods. 2014 Apr;199:124-8. doi:10.1016/j.jviromet.2014.01.013
13. Wang G, Zhou F, Buchy P, Zuo T, Hu H, Liu J, Song Y, Ding H, Tsai C, Chen Z, Zhang L, Deubel V, Zhou P. DNA Prime and Virus-like Particle Boost from a Single H5N1 Strain Elicits Broadly Neutralizing Antibody Responses against Head Region of H5 Hemagglutinin. J Infect Dis. 2014 Mar 1;209(5):676-85
14. Chen J, Yan B, Chen Q, Yao Y, Wang H, Liu Q, Zhang S, Wang H, Chen Z*. Evaluation of neutralizing efficacy of monoclonal antibodies specific for 2009 pandemic H1N1 influenza A virus in vitro and in vivo. Arch Virol. 2014 Mar;159(3):471-83
15. Zheng M, Luo J, Chen Z* Development of universal influenza vaccines based on influenza virus M and NP genes. Infection. 2013 Nov 1. [Epub ahead of print]
16. Chen J, Liu Q, Chen Q, Xiong C, Yao Y, Wang H, Wang H, Chen Z*. Comparative analysis of antibody induction and protection against influenza virus infection by DNA immunization with HA, HAe, and HA1 in mice. Arch Virol. 2013 Oct 17. [Epub ahead of print]
17. Wang B, Zhang H, Chen Q, Chen Z*. Full Genome Sequence of an Avian Influenza H5N1 Virus Isolated from the Environment in Hunan Province, China. Genome Announc. 2013 May 16;1(3). pii: e00088-13. doi: 10.1128/genomeA.00088-13.
18. Wang W, Cai J, Wu Y, Hu L, Chen Z, Hu J, Chen Z, Li W, Guo M, Huang Z. Novel activity of KRAB domain that functions to reinforce nuclear localization of KRAB-containing zinc finger proteins by interacting with KAP1. Cell Mol Life Sci. 2013 May 12. [Epub ahead of print]
19. Zhang F, Fang F, Chang H, Peng B, Wu J, Chen J, Wang H, Chen Z*. Comparison of protection against H5N1 influenza virus in mouse offspring provided by maternal vaccination with HA DNA and inactivated vaccine. Arch Virol. 2013 Jun;158(6):1253-65. doi: 10.1007/s00705-013-1621-y. Epub 2013 Feb 6.
20. Chen Q, Huang S, Chen J, Zhang S, Chen Z*. NA Proteins of Influenza A Viruses H1N1/2009, H5N1, and H9N2 Show Differential Effects on Infection Initiation, Virus Release, and Cell-Cell Fusion. PLoS One. 2013;8(1):e54334. doi: 10.1371/journal.pone.0054334. Epub 2013 Jan 22.
21. Huang Shengpin, Chen Jingjing, Chen Quanjiao, Wang Huadong, Yao Yanfeng, Chen Jian jun, Chen Ze*. A second CRM1-dependent nuclear export signal in the influenza A virus NS2 protein (NEP) mediates the nuclear export of viral ribonucleoproteins.
J Virol. 2013 Jan;87(2):767-78. doi: 10.1128/JVI.06519-11. Epub 2012 Oct 31.
22. Zheng D, Yi Y, Chen Z*. Development of Live-Attenuated Influenza Vaccines against Outbreaks of H5N1 Influenza.Viruses. 2012 Dec 10;4(12):3589-605. doi: 10.3390/v4123589. invited Review
23. Luo J, Zheng D, Zhang W, Fang F, Wang H, Sun Y, Ding Y, Xu C, Chen Q, Zhang H, Huang D, Sun B, Chen Z*. Induction of cross-protection against influenza A virus by DNA prime-intranasal protein boost strategy based on nucleoprotein. Virol J. 2012 Nov 23;9(1):286.
24. Yang Zhongdong, Wang Shilei, Li Wei, Li Changgui, Dong Jinrong, Li Fangjun, Wang Shuqiao, Chai Wenqing, Sun Bing, Chen Ze*. The long-term immunogenicity of an inactivated split-virion 2009 pandemic influenza A H1N1 vaccine: randomized, observer-masked, single-center clinical study. Results in Immunology. Results in Immunology. Available online 9 October 2012.
25. Wang X, Zhang W, Liu F, Zheng M, Zheng D, Zhang T, Yi Y, Ding Y, Luo J, Dai C, Wang H, Sun B, Chen Z*. Intranasal immunization with live attenuated influenza vaccine plus chitosan as an adjuvant protects mice against homologous and heterologous virus challenge. Arch Virol. 2012 Aug; 157(8):1451-61. Epub 2012 May 4.
26. Yao Yanfeng, Wang Huadong, Chen Quanjiao, Zhang Hongbo, Zhang Tao, Chen Jianjun, Xu Bing, Wang Hanzhong, Sun Bing, Chen Z* Characterization of low pathogenic H6N6 avian influenza viruses in central China. Arch Virol. 2013 Feb;158(2):367-77. doi: 10.1007/s00705-012-1496-3. Epub 2012 Oct 9.
27. Wang Ba, Chen Quanjiao and Chen Z* Complete Genome Sequence of a H9N2 Avian Influenza Virus Isolated from egret in Lake Dongting Wetland Journal of Virology. 2012 Nov; 86(21):11939.
28. Tao L, Chen J, Zheng Z, Meng J, Zhang Z, Chen Y, Luo H, Li H, Chen Z, Hu Q, Wang H. H5N1 influenza virus-like particles produced by transient expression in mammalian cells induce humoral and cellular immune responses in mice. Can J Microbiol. 2012 Apr;58(4):391-401. Epub 2012 Mar 19
29. Xu C, Hu WB, Xu K, He YX, Wang TY, Chen Z, Li TX, Liu JH, Buchy P, Sun B. Amino acids 473V and 598P of PB1 from an avian origin influenza A virus contribute to polymerase activity especially in mammalian cells. J Gen Virol. 2012 Mar;93(Pt 3):531-40. Epub 2011 Nov 16.
30. Zhang H, Chen Q, Chen Z*. Characterization of an H4N2 avian influenza virus isolated from domestic duck in Dongting Lake wetland in 2009. Virus Genes. 2012 Feb;44(1):24-31. Epub 2011 Aug 19.
31. Cui ZQ, Ren Q, Wei HP, Chen Z, Deng JY, Zhang ZP, Zhang XE. Quantum dot-aptamer nanoprobes for recognizing and labeling influenza A virus particles. Nanoscale. 2011 Jun;3(6):2454-7. doi: 10.1039/c1nr10218d. Epub 2011 Apr 21.
32. Zhang H, Xu B, Chen Q, Chen J, Chen Z *.Characterization of an H10N8 influenza virus isolated from Dongting lake wetland. Virol J. 2011 Jan 27;8(1):42.
33. Xu K, Ling ZY, Sun L, Xu Y, Bian C, He Y, Lu W, Chen Z, Sun B. Broad Humoral and Cellular Immunity Elicited by a Bivalent DNA Vaccine Encoding HA and NP Genes from an H5N1 Virus. Viral Immunol. 2011 Feb;24(1):45-56
34. Wei J, Yan B, Chen Z, Li T, Deng F, Wang H, Hu Z. Production and characterization of monoclonal antibodies against the hemagglutinin of H5N1 and antigenic investigation of avian influenza H5N1 viruses isolated from China.Can J Microbiol. 2011 Jan;57(1):42-8
35. Zhang Hongbo, Xu Bing, Chen Quanjiao, Chen Ze *.Characterization of H9N2 influenza viruses isolated from Dongting Lake wetland in 2007. Arch Virol. 2011 Jan;156(1):95-105.
36. Wu J, wang F, Fang F, Zhang W, Chang H, Zheng L and Chen Z*.Superior protection provided by a single dose of MF59-adjuvanted whole inactivated H5N1 influenza vaccine in type 1 diabetic mice. Arch Virol. 2011 Mar;156(3):387-95.
37. Xu K, Klenk C, Liu B, Keiner B, Cheng J, Zheng BJ, Li L, Han Q, Wang C, Li T, Chen Z, Shu Y, Liu J, Klenk HD, Sun B. Modification of Nonstructural Protein 1 of Influenza A Virus by SUMO1. J Virol. 2011 Jan;85(2):1086-98.
38. Sui Z, Chen Q, Fang F, Zheng M, Chen Z*. Cross-protection against influenza virus infection by intranasal administration of M1-based vaccine with chitosan as an adjuvant. Vaccine. 2010 Nov 10;28(48):7690-8.
39. He Y, Xu K, Keiner B, Zhou J, Czudai V, Li T, Chen Z, Liu J, Klenk HD, Shu YL, Sun B. Influenza A virus replication Induces Cell Cycle Arrest in G0/G1 Phase. J Virol. 2010 Dec;84(24):12832-40.
40. Chang H, Huang C, Wu J, Fang F, Zhang W, Wang F, Chen Z*. A single dose of DNA vaccine based on conserved H5N1 subtype proteins provides protection against lethal H5N1 challenge in mice pre-exposed to H1N1 influenza virus Virol J. 2010 Aug 21;7:197
41. Guo Lina, Zheng Mei, Ding Yahong, Li Dongmei, Yang Zhongdong, Wang Haiming, Chen Quanjiao, Sui Zhiwei, Fang Fang and Chen Ze *. Protection against multiple influenza A subtypes by intranasal administration of recombinant nucleoprotein. Arch Virol. 2010 Nov;155(11):1765-75.
42. Chen J, Huang S, Chen Z*. Human cellular protein nucleoporin hNup98 interacts with influenza A virus NS2/NEP protein and over-expression of its GLFG repeat domain can inhibit virus propagation. J Gen Virol. 2010 Oct;91(Pt 10):2474-84.
43. Chang H, Li X, Teng Y, Liang Y, Peng B, Fang F, Chen Z*. Comparison of Adjuvant Efficacy of Chitosan and Aluminum Hydroxide for Intraperitoneally Administered Inactivated Influenza H5N1 Vaccine. DNA Cell Biol. 2010 Sep;29(9):563-8.
44. Ze Chen*, Zhongdong Yang. Pandemic Influenza Vaccine Clinical Trial Abstract Minimum Information: Summary on H1N1 vaccine in China: state manufacturers. published on the World Health Organization (WHO) website (http://www.who.int/entity/vaccine_research/diseases/influenza/18_feb_Ze Chen. pdf) 2010，
45. Lu B, Huang Y, Huang L, Li B, Zheng Z, Chen Z, Chen J, Hu Q, Wang H. Effect of mucosal and systemic immunization with virus-like particles of severe acute respiratory syndrome coronavirus in mice. Immunology. 2010 Jun;130(2):254-61.
46. Wu J, Zhang F, Fang F, Chang H, Wang F, Yang Z, Sun B, Chen Ze*. Efficacy of inactivated vaccine against H5N1 influenza virus infection in mice with type 1 diabetes. Vaccine. 2010 Mar 24;28(15)：2775-81
47. Sui Z, Chen Q, Wu R, Zhang H, Zheng M, Wang H, Chen Ze*. Cross-protection against influenza virus infection by intranasal administration of M2-based vaccine with chitosan as an adjuvant. Arch Virol. Arch Virol. 2010 Apr;155(4):535-44
48. Liang XF, Wang HQ, Wang JZ, Fang HH, Wu J, Zhu FC, Li RC, Xia SL, Zhao YL, Li FJ, Yan SH, Yin WD, An K, Feng DJ, Cui XL, Qi FC, Ju CJ, Zhang YH, Guo ZJ, Chen PY, Chen Z,Yan KM, Wang Y. Safety and immunogenicity of 2009 pandemic influenza A H1N1 vaccines in China: a multicentre, double-blind, randomised, placebo-controlled trial.Lancet. 2010 Jan 2;375(9708):56-66.
49. Wu R, Guan Y, Yang Z, Chen J, Wang H, Chen Q, Sui Z, Fang F, Chen Z*. A live bivalent influenza vaccine based on a H9N2 virus strain.Vaccine. 2010 Jan 8;28(3):673-80.
50. Chen J, Fang F, Yang Z, Liu X, Zhang H, Zhang Z, Zhang X, Chen Z*.Characterization of highly pathogenic H5N1 avian influenza viruses isolated from poultry markets in central China. Virus Res. 2009 Dec;146(1-2):19-28.
51. Huang Shengping, Chen Jingjing, Wang Huadong, Sun Bing, Wang Hanzhong, Zhang Zhiping, Zhang Xianen, Chen Z*. Influenza A virus matrix protein 1 interacts with hTFIIIC102-s, a short isoform of the polypeptide 3 subunit of human general transcription factor IIIC. Arch Virol. 2009;154(7):1101-10.
52. Wu Rui, Chen Quanjiao, Zheng Liyun, Chen Jianjun, Sui Zhiwei, Chen Ze*.Generation and evaluation of an H9N1 influenza vaccine derived by reverse genetics that allows utilization of a DIVA strategy for control of H9N2 avian influenza. Arch Virol 2009;154(8):1203-10.
53. Zheng L, Wang F, Yang Z, Chen J, Chang H, Chen Z*. A single immunization with HA DNA vaccine by electroporation induces early protection against H5N1 avian influenza virus challenge in mice. BMC Infect Dis. 2009 Feb 12;9:17.
54. Chen J, Yang Z, Chen Q, Liu X, Fang F, Chang H, Li D, Chen Ze*. Characterization of H5N1 influenza A viruses isolated from domestic green-winged teal .Virus Genes. 2009 Feb;38(1):66-73.
55. Chen Q，Kuang H, Wang H, Fang F, Yang ZD, Zhang Zh, Zhang X, Chen Z*. Comparing the ability of a series of viral protein-expressing plasmid DNAs to protect against H5N1 influenza virus. Virus Genes. 2009, 38:30–38.
56. Lu B, Tao L, Wang T, Zheng Z, Li B, Chen Z, Huang Y, Hu Q, Wang H. Humoral and cellular immune responses induced by 3a DNA vaccines against severe acute respiratory syndrome (SARS) or SARS-like coronavirus in mice. Clin Vaccine Immunol. 2009 Jan;16(1):73-7.
57. Zhou Y, Fang F, Chen J, Wang H, Chang H, Yang Z, Chen Ze*. Electroporation at Low Voltages Enables DNA Vaccine to Provide Protection against a Lethal H5N1 Avian Influenza Virus Challenge in Mice. Intervirology. 2008 Sep 24;51(4):241-246.
58. Wang Fuyan, Yang Wen, Fang Fang, Chang Haiyan, Yu Ping, Chen Ze*. Essential sequence of influenza B virus hemagglutinin DNA to provide protection against lethal homologous viral infection. DNA and cell biology 2008 Jul;27(7):377-85.
59. Wang W, Cui ZQ, Han H, Zhang ZP, Wei HP, Zhou YF, Chen Z.Zhang XE.Imaging and characterizing influenza A virus mRNA transport in living cells. Nucleic Acids Res. 2008 Sep;36(15):4913-28.
60. Fang F., Cai XQ, Chang HY., Wang HD, Yang ZD, Chen Z*. Protection ability of influenza B virus DNA Vaccines expressing hamagglutinin, neuraminidase or both in mice .Acta virologica 52:107–112, 2008
61. Wu R, Sui ZW, Zhang HB, Chen QJ, Liang WW, Yang KL, Xiong ZL, Liu ZW, Chen Z*, Xu DP. Characterization of a pathogenic H9N2 influenza A virus isolated from central China in 2007. Arch Virol. 2008;153(8):1549-55
62. Bai B, Lu X, Meng J, Hu Q, Mao P, Lu B, Chen Z, Yuan Z, Wang H. Vaccination of mice with recombinant baculovirus expressing spike or nucleocapsid protein of SARS-like coronavirus generates humoral and cellular immune responses. Mol Immunol. 2008 Feb;45(4):868-75.
63. Chen J, Zhang F, Fang F, Chang H, Chen Z*. Vaccination with hemagglutinin or neuraminidase DNA protects BALB/c mice against influenza virus infection in presence of maternal antibody. BMC Infect Dis. 2007 Oct 16;7(1):118.
64. Chen Z*, Watanabe S. An intrastrain variation of a 189-bp repeat region in the human cytomegalovirus replication origin. Acta Virol. 2007;51(1):21-6.
65. Lu X, Chen Y, Bai B, Hu H, Tao L, Yang J, Chen J, Chen Z, Hu Z, Wang H. Immune responses against severe acute respiratory syndrome coronavirus induced by virus-like particles in mice. Immunology. 2007 Dec;122(4):496-502.
66. Hu H, Lu X, Tao L, Bai B, Zhang Z, Chen Y, Zheng F, Chen J, Chen Z, Wang H. Induction of specific immune responses by SARS-CoV spike DNA vaccine with or without interleukin-2 immunization using different immune routes in mice. Clin Vaccine Immunol. 2007 Jul;14(7):894-901.
67. Yu Y, Jin H, Chen Z, Yu QL, Ma YJ, Sun XL, Wang B. Children's vaccines do not induce cross reactivity against SARS-CoV. J Clin Pathol. 2007 Feb;60(2):208-11.
68. Qiu M, Fang F, Chen Y, Wang H, Chen Q, Chang H, Wang F, Wang H, Zhang R,Chen Z*，Protection against avian influenza H9N2 virus challenge by immunization with hemagglutinin- or neuraminidase-expressing DNA in BALB/c mice. Biochem Biophys Res Commun. 2006 May 19;343(4):1124-31.
69. Li X, Fang F, Song Y, Yan H, Chang H, Sun S, Chen Z*，Essential sequence of influenza neuraminidase DNA to provide protection against lethal viral infection. DNA Cell Biol. 2006 Apr;25(4):197-205.
70. Fenghua Zhang, Jianjun Chen, Fang Fang, Yu Zhou, Jian Wu, Haiyan Chang, Ran Zhang, Fuyan Wang, Xiaoman Li, Hanzhong Wang, Guohua Ma and Ze Chen*，Maternal immunization with both hemagglutinin- and neuraminidase-expressing DNAs provide an enhanced protection against a lethal influenza virus challenge in infant and adult mice. DNA Cell Biology. 2005; 24(11):758-765.
71. Guo Y, Sun S, Wang K, Zhang S, Zhu W, Chen Z. Elicitation of immunity in mice after immunization with the S2 subunit of the severe acute respiratory syndrome coronavirus. DNA Cell Biol. 2005 Aug;24(8):510-5.
72. Jianjun Chen, Fang Fang, Xiangzhong Li, Haiyan Chang and Chen Ze*. Protection against influenza virus infection in BALB/c mice immunized with a single dose of neuraminidase-expressing DNAs by electroporation. Vaccine. 2005;23(34):4322-4328.
73. Hu W, Bai B, Hu Z, Chen Z, An X, Tang L, Yang J, Wang H, Wang H. Development and evaluation of a multitarget real-time Taqman reverse transcription-PCR assay for detection of the severe acute respiratory syndrome-associated coronavirus and surveillance for an apparently related coronavirus found in masked palm civets. J Clin Microbiol. 2005 May;43(5):2041-6.
74. Zhu Q, Chang H, Chen Y, Fang F, Xue C, Zhang F, Qiu M, Wang H, Wang B, Chen Ze*. Protection of inactivated influenza virus vaccine against lethal influenza virus infection in diabetic mice. Biochem Biophys Res Commun. 2005 Apr 1;329(1):87-94.
75. Jin H, Xiao C, Chen Z, Kang Y, Ma Y, Zhu K, Xie Q, Tu Y, Yu Y, Wang B. Induction of Th1 type response by DNA vaccinations with N, M, and E genes against SARS-CoV in mice. Biochem Biophys Res Commun. 2005 Mar 25;328(4):979-86.
专著
1. 《分子病毒学》，流行性感冒病毒章节，黄文林主编，人民卫生出版，2002年，2005年再版
2. 《生物化学与分子生物学实验教程》， 第四章节， 约2万字， 梁宋平主编，高等教育出版社，2003年
3. 《疫苗研究与应用》，流感病毒疫苗章节，约4万字, 赵铠主编，人民卫生出版社，2013年
研究方向
1. 流感病毒及流感疫苗的分子生物学基础研究；
2. 流感病毒灭活疫苗及基因工程亚单位疫苗研究；
3. 基于反向遗传学技术的流感病毒减毒活疫苗研究；
4. 流感疫苗新型佐剂及其作用机理研究；
5. 基于细胞大规模培养的流感疫苗新技术与新工艺开发。
