研究方向、内容及意义 课题组主要致力于植物发育与免疫应答的细胞信号网络研究。植物细胞骨架，包括微管（Microtubules, MTs）与微丝（Actin Filaments, F-actin），与细胞质膜以及细胞壁组成一个植物细胞特有的、高度动态的细胞骨架-质膜-细胞壁连续体（Cytoskeleton- Plasmamembrane-Cell Wall Continuum）。基于细胞骨架-质膜-细胞壁连续体，植物细胞形成了复杂而有序的细胞信号网络，时刻响应发育与环境信号，进行活跃的细胞骨架重排与细胞壁重构、细胞区室化（Cellular compartmentation）与细胞内稳态（Cellular homeostasis）重建，从而保障植物完成发育进程或者适应环境变化。特别是在植物与微生物相互作用过程中，细胞骨架-质膜-细胞壁连续体作为生物互作的第一道屏障（或免疫防线），在宿主与菌相互作用界面（Plant-Microbe Interface）形成过程中发挥至关重要作用，同时也是微生物（病原菌或共生菌）分泌的效应蛋白或毒力因子的重要靶标。 课题组主要有两个研究方向： 1. 细胞骨架-质膜-细胞壁连续体时空调控植物细胞形态建成 以模式植物拟南芥和重要经济作物棉花为研究对象，研究植物细胞形态建成中细胞骨架-质膜-细胞壁连续体的时空调控机制。重点研究细胞骨架在棉花纤维细胞伸长及次生细胞壁加厚过程中的调控作用，为改良棉花纤维品质提供重要的理论指导。 2. 植物-微生物互作的细胞信号网络 一方面，研究植物—黄萎病菌相互作用的细胞信号网络。以植物（包括棉花与拟南芥）—大丽轮枝菌互作体系为模型，研究揭示植物黄萎病致病的细胞分子机制。主要研究菌所分泌的效应蛋白如何通过菌与宿主间的相互作用界面转运至宿主细胞，进而操纵宿主细胞骨架系统，干扰囊泡运输，影响细胞壁结构与组成，抑制免疫反应。该研究对于阐明植物抗黄萎病机制具有重要意义。 另一方面，研究豆科植物—根瘤菌共生固氮的细胞信号网络。以大豆和苜蓿为研究对象，研究共生和植物先天免疫之间对话（cross-talk）的细胞分子机制。主要研究根瘤菌分泌的效应蛋白对共生固氮的调控机制，共生固氮信号的时空调控及长距离传导机制等。本研究对于通过选育高效固氮新种质或创制高效固氮新菌株而提高共生固氮效率具有重要意义。

发表论文(#Co-First Author; *Corresponding Author) 2012年回国后发表的论文： Guangda Wang, Changfeng Wang, Weiwei Liu, Li Dong, Juan Tian, Yanjun Yu, and Zhaosheng Kong*. (2018). Augmin antagonizes katanin at microtubule crossovers to control the dynamic organization of plant cortical arrays. Current Biology, 28: 1311-1317 Zhidi Feng, Juan Tian, Libo Han, Yuan Geng, Jie Sun*, and Zhaosheng Kong*. (2018). The Myosin5-mediated actomyosin motility system is required for Verticillium pathogenesis of cotton. Environmental Microbiology, 20:1607-1621（Cover story） Shenghao Zou, Huan Wang, Yiwen Li, Zhaosheng Kong, and Dingzhong Tang*. (2018). The NB-LRR gene Pm60 confers powdery mildew resistance in wheat. New Phytologist, 218: 298-309 Changfeng Wang#, Weiwei Liu#, Guangda Wang#, Jun Li, Li Dong, Libo Han, Qi Wang, Juan Tian, Yanjun Yu, Caixia Gao, and Zhaosheng Kong*.(2017). KTN80 confers precision to microtubule severing by specific targeting of katanin complexes in plant cells. EMBO Journal, 36: 3435-3447 Yujia Leng#, Yaolong Yang#, Deyong Ren#, Lichao Huang, Liping Dai, Yuqiong Wang, Long Chen, Zhengjun Tu, Yihong Gao, Xueyong Li, Li Zhu, Jiang Hu, Guangheng Zhang, Zhenyu Gao, Longbiao Guo, Zhaosheng Kong, Yongjun Lin, Qian Qian*, and Dali Zeng*.(2017). A rice PECTATE LYASE-LIKE Gene is required for plant growth and leaf senescence. Plant Physiology, 174:1151-1166 Jiliang Hu#, Huanjie Yang#, Jinye Mu, Tiancong Lu, Juli Peng, Xian Deng, Zhaosheng Kong, Shilai Bao, Xiaofeng Cao, and Jianru Zuo*. (2017). Nitric oxide regulates protein methylation during stress responses in plants. Molecular Cell, 67:702-710 Shundai Li, Logan Bashline, Yunzhen Zheng, Xiaoran Xin, Shixin Huang, Zhaosheng Kong, Seong H. Kim, Daniel J. Cosgroveb, and Ying Gu*. (2016). Cellulose synthase complexes act in a concerted fashion to synthesize highly aggregated cellulose in secondary cell walls of plants. Proc Natl Acad Sci U S A. 113: 11348-11353 Libo Han#, Yuanbao Li#, Yongduo Sun, Haiyun Wang, Zhaosheng Kong, and Guixian Xia*. (2016) The two domains of cotton WLIM1a protein are functionally divergent. Science China Life Sciences, 59：206-212 Liangliang Chen#, Yuancheng Peng#, Juan Tian, Xiaohong Wang, Zhaosheng Kong, Tonglin Mao, Ming Yuan, and Yunhai Li. (2016). TCS1, a microtubule-binding protein, interacts with KCBP/ZWICHEL to regulate trichome cell shape in Arabidopsis thaliana. PLOS Genetics, 12:e1006266 Juan Tian, Libo Han, Zhidi Feng, Guangda Wang, Weiwei Liu, Yinping Ma, Yanjun Yu, and Zhaosheng Kong*. (2015). Orchestration of microtubules and the actin cytoskeleton in trichome cell shape determination by a plant-unique kinesin. eLIFE, 4: eLife09351 Guangheng Wu, Simu Liu, Yaofei Zhao, Wei Wang, Zhaosheng Kong, and Dingzhong Tang*. (2015). ENHANCED DISEASE RESISTANCE4 Associates with CLATHRIN HEAVY CHAIN2 and Modulates Plant Immunity by Regulating Relocation of EDR1 in Arabidopsis. Plant Cell, 27: 857-873 Zhaosheng Kong, Motohide Ioki, Siobhan Braybrook, Shundai Li, Zheng-Hua Ye, Yuh-Ru Julie Lee, Takashi Hotta, Anny Chang, Juan Tian, Guangda Wang and Bo Liu*. (2015). Kinesin-4 Functions in Vesicular Transport on Cortical Microtubules and Regulates Cell Wall Mechanics during Cell Elongation in Plants. Molecular Plant, 7: 1011-1023 Fenni Lv, Haihai Wang, Xinyu Wang, Libo Han, Yinping Ma, Sen Wang, Zhidi Feng, Xiaowei Niu, Caiping Cai, Zhaosheng Kong, Tianzhen Zhang and Wangzhen Guo*. (2015). GhCFE1A, a dynamic linker between the ER network and actin cytoskeleton, plays an important role in cotton fire cell initiation and elongation. Journal of Experimental Biology, 7: 1877-1989 Ting Liu#, Juan Tian#, Guangda Wang#, Yanjun Yu, Chaofeng Wang, Yinping Ma, Xiaxia Zhang, Guixian Xia, Bo Liu, and Zhaosheng Kong*. (2014) Augmin Triggers Microtubule-dependent Microtubule Nucleation in Interphase Plant Cells. Current Biology, 24: 2708-2713 Libo Han, Yuanbao Li, Haiyun Wang, Xiaomin Wu, Chunli Li, Ming Luo, Shenjie Wu, Zhaosheng Kong, Yan Pei, Gaili Jiao, and Gui-xian Xia. (2013) The Dual Functions of WLIM1a in Cell Elongation and Secondary Wall Formation in Developing Cotton Fibers. Plant Cell, 11: 4421-4438. 回国以前发表的论文： Takashi Hotta, Zhaosheng Kong, Chin-Min Ho, Cui-jing Tracy Zeng, Tetsuya Horio, Sophia Fong, Trang Vuong, Yu-Rue Julie Lee, and Bo Liu. (2012). Characterization of the Arabidopsis Augmin Complex Uncovers Its Critical Function in the Assembly of the Acentrosomal Spindle and Phragmoplast Microtubule Arrays. Plant Cell, 24: 1494-1509. Chin-Min Ho#, Takashi Hotta#, Zhaosheng Kong#, Cuijing Tracy Zeng#, Jie Sun, Yu-Rue Lee, and Bo Liu. (2011). Augmin plays a critical role in organizing the spindle and phragmoplast microtubule arrays in Arabidopsis. Plant Cell, 23: 2606-2618. Zhaosheng Kong, Takashi Hotta, Y.-R. Julie Lee, Tetsuya Horio and Bo Liu. (2010). The γ-tubulin complex protein GCP4 is required for organizing functional microtubule arrays in Arabidopsis thaliana. Plant Cell, 22: 191–204. (Cover story) Longbiao Guo#, Chin-Min Ho#, Zhaosheng Kong#, Yu-Rue Lee, Qian Qian and Bo Liu. (2009). Evaluating the microtubule cytoskeleton and its interacting proteins in monocots by mining the rice genome. Annals of Botany, 103: 387-402. Wenqiang Yang, Zhaosheng Kong, Edith Omo-Ikerodah, Wenying Xu, Qun Li and Yongbiao Xue. (2008). Calcineurin B-like interacting protein kinase OsCIPK23 functions in pollination and drought stress responses in rice (Oryza sativa L.). Journal of Genetics and Genomics, 35: 531-543. Meina Li, Wenying Xu, Wenqiang Yang, Zhaosheng Kong and Yongbiao Xue. (2007). Genome-wide gene expression profiling reveals conserved and novel molecular functions of the stigma in rice. Plant Physiology, 144: 1797-812. Zhaosheng Kong, Wenying Xu, Qun Li and Yongbiao Xue. (2007). Identification, expression and functional analysis of a receptor-like cytoplasmic kinase, OsRLCK1, in rice (Oryza sativa L.). Journal of Integrative Plant Biology, 49: 898-907. Zhaosheng Kong, Meina Li, Wenqiang Yang, Wenying Xu and Yongbiao Xue. (2006). A Novel Nuclear-Localized CCCH-type Zinc Finger Protein, OsDOS, Is Involved in Delaying Leaf Senescence in Rice (Oryza sativa L.). Plant Physiology, 141: 1376–1388. Lefu Lan#, Meina Li#, Ying Lai#, Wenying Xu, Zhaosheng Kong, Kai Ying, Bin Han and Yongbiao Xue. (2005). Microarray analysis reveals similarities and variations in genetic programs involved in pollination/fertilization and stress responses in rice (Oryza sativa L.). Plant Molecular Biology, 59: 151–164. Lefu Lan#, Wei Chen#, Ying Lai, Jinfeng Suo, Zhaosheng Kong, Can Li, Ying Lu, Yujun Zhang, Xiangyu Zhao, Xiansheng Zhang, Yansheng Zhang, Bin Han, Jing Cheng and Yongbiao Xue. (2004). Monitoring of gene expression profiles and isolation of candidate genes involved in pollination and fertilization in rice (Oryza sativa L.) with a 10K cDNA microarray. Plant Molecular Biology, 54: 471–487.